Jan Liphardt

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Jan Liphardt
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Born1972 (age 51–52)
CitizenshipGermany
Awards
  • Searle Scholar (2006)
  • Sloan Research Fellowship
  • Hellman Fellow
Academic background
EducationBiophysics
Alma materCambridge University
University of California, Berkeley
Academic advisorsCarlos Bustamante, Ignacio Tinoco Jr.
Academic work
DisciplineBiological engineering
Biophysics
Microscopy
Cell biology
InstitutionsUniversity of California, Berkeley
Stanford University
Notable studentsAleksandra Radenovic
Main interestsSingle molecule biophysics
Solid State Nanopores
Optical tweezers
Super-resolution microscopy
Websitehttps://liphardtlab.stanford.edu

Jan Liphardt (born in 1972 in Germany) is a German biophysicist. His research primarily focuses on single molecule biophysics. As a postdoctoral fellow in the labs of Carlos Bustamante and Ignacio Tinoco Jr. in the physics department at University of California, Berkeley, he developed technologies that allowed single RNA molecules to grabbed and mechanically unfolded.[1] Subsequent work includes the development of "Plasmon Rulers"[2] (with Paul Alivisatos), the use of super-resolution imaging to understand the organization of microbial biofilms[3] (with Steven Chu), and the use of photo activation localization microscopy to characterize the organization of single transmembrane proteins[4] (with Eric Betzig). He is an associate professor of Bioengineering at Stanford University. His research has been cited more than 15,000 times.[5]

Mechanical Manipulation of Single Nucleic Acids

In the original work performed in 2001[1], single RNA hairpins were attached to long RNA/DNA 'handles' which were chemically coupled to beads held by optical tweezers. The RNA/DNA handles allow nanometer scale movements (and piconewton range forces) to be applied and measured. This basic tool geometry has been cited by more than 1100 papers covering RNA structure/function (reviewed in[6][7][8]), studies of thermodynamics of small perturbed systems (reviewed in[9][10]), and studies of molecular machines that act on RNA, such as RNA helicases[11] (reviewed in[12]). The original work (and followup studies) are used in life sciences and nanotechnology textbooks to illustrate the energetics and kinetics of nucleic acid folding.[13][14][15]

NCI PS-OC Program

In 2009, Liphardt became the principal investigator of one of 12 national physical science oncology centers,[16][17] which were intended to bring physical scientists to cancer research.[18][19]

Biden Cancer Moonshot

In 2016, as part of the Beau Biden Cancer Moonshot, he led a team of scientists and volunteers from a Bay Area social network[20] to build a system for cancer patients to share their healthcare data with teachers, researchers, and journalists.[21]

References

  1. ↑ 1.0 1.1 Liphardt J, Onoa B, Smith SB, Tinoco I, Bustamante C (2001). "Reversible unfolding of single RNA molecules by mechanical force". Science. 292 (5517): 733–7. Bibcode:2001Sci...292..733L. doi:10.1126/science.1058498. PMID 11326101.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  2. ↑ Sönnichsen C, Reinhard BM, Liphardt J, Alivisatos AP (2005). "A molecular ruler based on plasmon coupling of single gold and silver nanoparticles". Nat Biotechnol. 23 (6): 741–5. doi:10.1038/nbt1100. PMID 15908940.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  3. ↑ Berk V, Fong JC, Dempsey GT, Develioglu ON, Zhuang X, Liphardt J; et al. (2012). "Molecular architecture and assembly principles of Vibrio cholerae biofilms". Science. 337 (6091): 236–9. Bibcode:2012Sci...337..236B. doi:10.1126/science.1222981. PMC 3513368. PMID 22798614.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  4. ↑ Greenfield D, McEvoy AL, Shroff H, Crooks GE, Wingreen NS, Betzig E; et al. (2009). "Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy". PLOS Biol. 7 (6): e1000137. doi:10.1371/journal.pbio.1000137. PMC 2691949. PMID 19547746.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  5. ↑ "Liphardt Citations". Google Scholar. Retrieved 2023-12-27.
  6. ↑ Bustamante CJ, Chemla YR, Liu S, Wang MD (2021). "Optical tweezers in single-molecule biophysics". Nat Rev Methods Primers. 1. doi:10.1038/s43586-021-00021-6. PMC 8629167. PMID 34849486.
  7. ↑ Tinoco I, Chen G, Qu X (November 2010). "RNA reactions one molecule at a time". Cold Spring Harb Perspect Biol. 2 (11): a003624. doi:10.1101/cshperspect.a003624. PMC 2964187. PMID 20739416.
  8. ↑ Li PT, Vieregg J, Tinoco I (2008). "How RNA unfolds and refolds". Annu Rev Biochem. 77: 77–100. doi:10.1146/annurev.biochem.77.061206.174353. PMID 18518818.
  9. ↑ Rissone P, Ritort F (July 2022). "Nucleic Acid Thermodynamics Derived from Mechanical Unzipping Experiments". Life (Basel). 12 (7): 1089. Bibcode:2022Life...12.1089R. doi:10.3390/life12071089. PMC 9320087. PMID 35888177.
  10. ↑ Ritort F (August 2006). "Single-molecule experiments in biological physics: methods and applications". J Phys Condens Matter. 18 (32): R531–83. arXiv:cond-mat/0609378. doi:10.1088/0953-8984/18/32/R01. PMID 21690856.
  11. ↑ Dumont S, Cheng W, Serebrov V, Beran RK, Tinoco I, Pyle AM, Bustamante C (January 2006). "RNA translocation and unwinding mechanism of HCV NS3 helicase and its coordination by ATP". Nature. 439 (7072): 105–8. Bibcode:2006Natur.439..105D. doi:10.1038/nature04331. PMC 1560093. PMID 16397502.
  12. ↑ Ritchie DB, Woodside MT (October 2015). "Probing the structural dynamics of proteins and nucleic acids with optical tweezers". Curr Opin Struct Biol. 34: 43–51. doi:10.1016/j.sbi.2015.06.006. PMC 7126019. PMID 26189090.
  13. ↑ Nelson, Philip; Goodsell, David S. (2014). Biological physics: energy, information, life; with new art by David Goodsell. New York, NY: Freeman. ISBN 978-0-7167-9897-2.
  14. ↑ Walter, Nils; Woodson, Sarah A.; Batey, Robert T. (2008-11-18). Non-Protein Coding RNAs. Berlin: Springer Science & Business Media. ISBN 978-3-540-70840-7.
  15. ↑ Bai, Y.L.; Zheng, Q.S.; Wei, Y.G. (2007-04-05). IUTAM Symposium on Mechanical Behavior and Micro-Mechanics of Nanostructured Materials. Dordrecht London: Springer Science & Business Media. ISBN 978-1-4020-5624-6.
  16. ↑ "UCSF Scientists Help Guide Cancer Research to a Melding of Physics & Biology". UCSF. November 4, 2009. Retrieved December 28, 2023.
  17. ↑ "Cal, UCSF scientists form cancer research unit". SFGATE. October 27, 2009. Retrieved December 28, 2023.
  18. ↑ Yarris, Lynn (March 11, 2009). "Berkeley Scientists Find New Way to Get Physical in the Fight Against Cancer". Lawrence Berkeley National Lab. Retrieved December 28, 2023.
  19. ↑ Barker, Anna (March 23, 2010). "National Cancer Institute Research: Today's Progress; Tomorrow's Challenges" (PDF). NCI. Retrieved December 28, 2023.
  20. ↑ "FACT SHEET: At Cancer Moonshot Summit, Vice President Biden Announces New Actions to Accelerate Progress Toward Ending Cancer As We Know It". The White House, Office of the Press Secretary. June 28, 2016. Retrieved December 28, 2023.
  21. ↑ Suarez, Ray (January 12, 2017). "The 'Cancer Moonshot,' One Year After Takeoff". KQED/NPR. Retrieved December 28, 2023.

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